PML-RARa

The SensiQuant Direct PML/RARa products are designed for the QUALITATIVE detection and QUANTITATIVE measurement of BCR1, BCR2, and BCR3 transcripts in cases of Acute Promyelocytic Leukemia (APL).

The product line includes:

• SensiQuant Direct Master Mix
• SensiQuant Standard (from 10^5 to 10 copies)
• SensiQuant Positive Control
• Lyoset Reference (lyophilized cells, from 10% to 0.1% + Negative Control)

Main Breakpoints in the Cluster

PML/RARa t(15;17), variant BCR1
PML/RARa t(15;17), variant BCR2
PML/RARa t(15;17), variant BCR3

Application

• QUALITATIVE Analysis = SensiQuant Direct Master Mix + SensiQuant Positive Control
• QUANTITATIVE Analysis = SensiQuant Direct Master Mix + SensiQuant Standard

• Lyoset Reference: Reference material consisting of ampoules containing lyophilized mixtures with known quantities of cells positive for the targeted translocation, diluted in negative cells. Essential for qualitative or quantitative performance assessment of translocation detection assays.

Benefits

• One-Step Technology
• Single plasmid of PML/RARa and ABL
• Linear Range (1,000,000 to 10 copies PML/RARa)
• Consistent Thermal Cycle across the SensiQuant DIRECT product line
• High ABL Copy Numbers (enhanced method sensitivity)
• High specificity, sensitivity, and accuracy
• High reproducibility (intra- and inter-assay)
• Fast and easy to use
• High stability
• CE/IVD marking*

*except for LRF-045